Cell culture is the most core and fundamental technology in biotechnology; A culture dish is a laboratory vessel used for culturing microorganisms or cells. One of the important indicators of biological cell culture is adhesion and extension. Although adhesion and extension are the basic growth characteristics of most cultured cells in vitro, some cells have poor adhesion and extension characteristics during growth, such as lymphocytes. Therefore, it is necessary to require the cell surface of the in vitro cell culture device to have a certain degree of hydrophilicity in order to meet the requirements of adherent cell in vitro culture. However, cell culture dishes in existing technologies, such as polystyrene (PS), polyethylene (PE), polycarbonate (PC), etc., have surfaces that are not hydrophilic, making them very difficult for adherent cell culture.

Therefore, there is a need for a treatment method that can modify the surface of cell culture dishes to make them hydrophilic.

In order to improve the adhesion characteristics and enhance the stability of biological cell culture dishes, plasma surface treatment can be used to increase the hydrophilicity of cell culture dishes, thereby improving cell growth.

Plasma surface treatment of petri dishes

Plasma is the fourth fundamental form of matter besides solids, liquids, and gases, composed of a large number of charged particles in an unbound state.

Plasma surface treatment mainly involves high-frequency discharge ionization of gas molecules in the atmosphere medium to generate a large number of high-energy electrons, positively and negatively charged active particles, and free radicals, thereby modifying the materials placed in them.

The particle energy in plasma is between 0-20eV, while the material of cell culture dishes is mostly plastic polymers, with most of the bond energies in polymers between 0-10eV. Therefore, when plasma acts on the surface of cell culture dishes, it can cause the original chemical bonds on the surface of the dish to break, and the free radicals in the plasma form a network like cross-linked structure with these broken chemical bonds, greatly activating their surface activity. And when the plasma acts on the surface of the cell culture dish, it can remove the original pollutants and impurities on the surface of the cell culture dish.

In terms of organizational culture materials, plasma surface treatment technology has great industrial value. This treatment greatly improves the cell culture ability of the culture dish, and the aging of the treated culture dish does not have a significant impact on their ability to support cell growth. And the plasma surface treatment equipment itself is a very environmentally friendly equipment, which does not produce any pollution, and the treatment process also does not produce any pollution.